product source myc tag ca rac1 mouse  (Cell Signaling Technology Inc)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: List of Primary Antibodies Used in Immunohistochemistry

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Marker, DNA Synthesis

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Constitutively active RAC1 expression in transgenic mice. (A) Western blotting with anti-RAC1 (endogenous RAC1 and transgenic CA RAC1) and anti-MYC-Tag (transgenic CA RAC1-specific) antibodies at P4, P6, and P11 indicated that CA RAC1 expression in the retinas increased with age in both LO and HI transgenic lines. Constitutively active RAC1 protein bands are yellow due to an overlap of anti-RAC1 (red) and anti-MYC-Tag (green) antibodies. (B) Fluorescence values of the CA RAC1 band in each sample at P6 were normalized to fluorescence values of corresponding endogenous RAC1 band. Quantification of CA RAC1 expression is represented as a percentage of endogenous RAC1 levels in CA RAC1 retinas, shown by the bar graph (mean ± SEM, n = 2).

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Expressing, Transgenic Assay, Western Blot, Fluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Time course of abnormal outer nuclear layer (ONL) lamination in CA RAC1 retinas during retinal development. (A) Retinal sections from CA RAC1 (LO and HI) and nontransgenic WT (Control) littermates at several postnatal times (P4, P6, P11, P21, and P3m) stained with hematoxylin and eosin showed progressive defects in the ONL lamination of CA RAC1 retinas. At P4, CA RAC1 retinas (b, c) had apparently normal neuroblastic layers (NBL). At P6, the IS appeared in both control (d) and CA RAC1 (e, f) retinas, but some nuclei were mislocalized into the space between the RPE and IS layer in CA RAC1 retinas (e, f) (black arrows). The OPL formed closer to the apical margin of the ONL than in the control, leading to a thin ONL, particularly in the CA RAC1 HI line (f). At P11, additional nuclei were displaced into the presumptive subretinal space adjacent to the RPE in both transgenic lines (h, i). ONL nuclei aberrantly formed retinal folds or whorls in the central retinal region. Yellow arrows indicate the areas bridging ONL to INL with incomplete separation by the OPL. By P21, both LO and HI lines had fewer ONL nuclei (k, l), compared with the WT control (j). By 3 months (P3m), the ONL was reduced to a single layer of nuclei in the HI line (o) and two to three rows in the LO line (n). Scale bar: 50 μm. (B) Retinal sections of nontransgenic control (Control) and CA RAC1 littermates at P24 with TUNEL assay showed increased apoptosis signals (red) at ONL in CA RAC1 retinas (q), compared to the control (p). Scale bar: 20 μm. IPL, inner plexiform layer, GCL, ganglion cell layer.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Control, Staining, Transgenic Assay, TUNEL Assay

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Mislocalization of rod and cone photoreceptors in CA RAC1 retinas. Retinal sections of nontransgenic control and CA RAC1 mice were double immunostained with antibodies against MYC-Tag (green, CA RAC1) and rhodopsin (red) (A) or cone arrestin (red) (B). Retinal nuclei were labeled with DAPI (gray or blue). (A) Representative DAPI and merged images are shown. Mislocalized cells in the subretinal space in CA RAC1 retinas at P6 ([c–e], boxed regions) and at P11 ([h–j], boxed regions) were positive for CA RAC1 (green) and rhodopsin (red) double immunostaining (higher magnification images of boxed regions in [d] and [i] are shown in [e] and [j], respectively), indicating that these mislocalized cells were rod photoreceptors. Many more cells double-labeled with CA RAC1 (green) and rhodopsin (red) antibodies were mislocalized in the INL at P6 (d) and P11 (i), compared to the control (b, g). (a–d, f–i) Scale bar: 20 μm; (e, j) scale bar: 5 μm. (B) Merged immunostaining images of CA RAC1 (green) and cone arrestin (red) are shown in the control (k) and CA RAC1 (l) retinas at P24. Cone arrestin–positive somas were located at the apical surface of the ONL in the control, whereas cone arrestin–stained cell bodies were mislocalized in the CA RAC1 retina ([l], white arrows). (k, l) Scale bar: 10 μm. A magnified region of the white box in (l) is shown in (m, n). Dotted outlines in (m, n) display the location of cone arrestin–positive cell bodies, indicating that the cone cells were negative for CA RAC1 staining in CA RAC1 retinas. Scale bar: 2.5 μm.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Control, Labeling, Double Immunostaining, Immunostaining, Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Characterization of mislocalized rod cells in the presumptive subretinal space adjacent to the RPE in CA RAC1 retinas. (A) Rod photoreceptor IS and OS were immunostained with antibodies against retinoschisin (RS, green) and rhodopsin (red), respectively, at P11 and P21. In the CA RAC1 retina (b, d), the mislocalized photoreceptors in the subretinal space (white arrows) did not show any normally oriented RS (green) and rhodopsin (red) labeling. Scale bar: 20 μm. (B) TEMs of photoreceptor cells are shown for nontransgenic control (e) and CA RAC1 (f) retinas at P21. No IS/OS structure was found between mislocalized cells (R, C) and the RPE (f). Note that mislocalized cells adjacent to the RPE included rods (R, large clumps of heterochromatin typical of rod nuclei) and cones (C, one or more small clumps of heterochromatin surrounded by a large amount of lighter staining euchromatin characteristic of cone nuclei). Scale bars: 5 μm. (C) Photoreceptor synaptic terminals were detected by immunostaining with synaptophysin (SYN, red), a presynaptic marker, and protein kinase C alpha (PKCα, green), a rod bipolar cell marker. In comparison to the nontransgenic control (g) in which a continuous band of staining with SYN and PKCα was seen across OPL synaptic terminals, the CA RAC1 retina showed a fragmented staining pattern (h). In the CA RAC1 retina, ectopic staining of SYN was observed around apically mislocalized cells that was not associated with PKCα-stained rod bipolar cells ([h], white arrows) and PKCα-positive bipolar cell process protruded into the ONL to contact synaptic terminals ([h], yellow arrow). Scale bar: 10 μm. (D) TEM images of synaptic ribbons (SR) are shown in the nontransgenic control ([i], red arrow) and in a mislocalized cell adjacent to the RPE in the CA RAC1 retina ([j], red arrow). A higher magnification image of SR in (j) is shown in (k) (red arrow). Note that, in the CA RAC1 retina, a displaced ribbon tethered multiple vesicles and pointed toward the RPE. No horizontal (H) or bipolar (B) cells were found around the ribbon. N, nucleus. (i) Scale bar: 500 nm; (j) scale bar: 2 μm; (k) scale bar: 100 nm.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Labeling, Control, Staining, Immunostaining, Marker, Comparison

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Localization of retinal progenitor cells and migration of newly differentiated photoreceptors in CA RAC1 retinas. (A) Retinal sections of CA RAC1 and nontransgenic littermates (Control) at P6 immunostained with the antibody against pH3 (red, [a, b, e, f]) or Ki-67 (red, [c, d, g, h]). Nuclei were counterstained with DAPI. Images of central and periphery regions show similar staining patterns of pH3 and Ki-67 in control (a–d) and CA RAC1 (e–h) retinas. Note that no pH3- or Ki-67-positive labeling was detected within the mislocalized nuclei adjacent to the RPE ([e] and [g], white arrows). (B) The movement of BrdU-labeled cells in CA RAC1 retinas. Immunofluorescence images of BrdU-labeled cells (red) in nontransgenic control (i–k) and CA RAC1 (l–n) retinas are shown at 3 hours (P1), 4 days (P5), and 10 days (P11) after BrdU injection at P1. In CA RAC1 retinas, more mislocalized cells in the subretinal space (indicated by white arrows in [m] and [n]) were labeled by BrdU (red) at P11 (n) than at P5 (m). (o) Double staining of BrdU (red, stains the nucleus) and rhodopsin (green, stains the plasma membrane) indicates that BrdU-positive mislocalized cells in the subretinal space also stained for rhodopsin. The inset in (o) is higher magnification of the white box region. (i–o) Scale bar: 20 μm. Scale bar in the inset: 5 μm.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Migration, Control, Staining, Labeling, Immunofluorescence, Injection, Double Staining, Clinical Proteomics, Membrane

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Disrupted localization of the PAR6 complex in the CA RAC1 retina. (A) Retinal extracts (Extracts) of CA RAC1 and nontransgenic WT (Control) littermates at P11 were immunoprecipitated using either anti-RAC1 (endogenous RAC1 and transgenic CA RAC1, RAC1-IP) or anti-MYC-Tag (transgenic CA RAC1-specific, MYC-IP) antibody and control IgG (IgG-IP). Co-immunoprecipitated proteins were analyzed by Western blotting. PAR6 was co-IP in both RAC1-IP and MYC-IP immunocomplexes of CA RAC1 retinal extracts, but only in RAC1-IP immunocomplex of the control. (B) Retinal sections of CA RAC1 mice at P11 were double immunostained with antibodies against MYC-Tag (green, CA RAC1) and PAR6 (red). Nuclei were labeled with DAPI (blue). Merged images indicate that CA RAC1 colocalized with PAR6. Scale bar: 20 μm. (C) Retinal sections of nontransgenic control and CA RAC1 littermates at P11 were immunostained with antibodies against components of the PAR6 complex (PAR6, aPKCλ, and PAR3) and β-catenin, an OLM marker. Immunostaining pattern of these proteins was disrupted and fragmented in CA RAC1 retinas. Scale bar: 20 μm.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Control, Immunoprecipitation, Transgenic Assay, Western Blot, Co-Immunoprecipitation Assay, Labeling, Marker, Immunostaining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Transgenic Expression of Constitutively Active RAC1 Disrupts Mouse Rod Morphogenesis

doi: 10.1167/iovs.13-13649

Figure Lengend Snippet: Constitutively active RAC1 forms a complex with dynein in the CA RAC1 retina. (A) Retinal sections of CA RAC1 mice at P11 were immunostained with anti-rhodopsin antibody (gray or red). Nuclei were labeled with DAPI (blue). Rhodopsin-stained and merged images are shown. Mislocalized cells with nuclei in the INL extended long processes toward the ONL (indicated by arrows). Scale bar: 20 μm. (B) Retinal extracts (Extracts) of CA RAC1 mice at P11 were immunoprecipitated using either anti-RAC1 (RAC1-IP) or anti-MYC-tag (MYC-IP) antibody and control IgG (IgG-IP). Dynein intermediate chain (DIC), not kinesin heavy chain (KHC), was detected in both RAC1-IP and MYC-IP immunocomplexes by Western blotting. (C) Retinal sections of CA RAC1 mice at P11 were double immunostained with antibodies against MYC-Tag (green, CA RAC1) and DIC (red). Individual and merged images are shown, indicating that CA RAC1 colocalized with DIC. Scale bar: 10 μm.

Article Snippet: Examination was done on three to four animals for each genotype per age group (P6, P11, or P24). table ft1 table-wrap mode="anchored" t5 caption a7 Name Target Host Dilution Product Source MYC-Tag CA RAC1 Mouse 1:2,000 2276; Cell Signaling Technology, Inc., Danvers, MA, USA Rabbit 1:400 2272; Cell Signaling Technology, Inc. Cone arrestin Cone Rabbit 1:10,000 AB15282; Millipore, Temecula, CA, USA Rhodopsin Rod Mouse 1:10,000 O 4886; Sigma-Aldrich, St. Louis, MO, USA Retinoschisin IS marker Rabbit 1:1,000 Takada et al. 57 Synaptophysin Presynaptic marker Mouse 1:200 S 5768; Sigma-Aldrich PKCα Rod bipolar cell Rabbit 1:1,000 P 4334; Sigma-Aldrich PH3 Mitosis marker Rabbit 1:100 06-570; Millipore Ki67 Progenitor cell marker Mouse 1:400 556003; BD Biosciences, San Jose, CA, USA BrdU DNA synthesis marker Rat 1:100 Ab6326; Abcam, Inc., Cambridge, MA, USA PAR6 Polarity protein Rabbit 1:100 Sc-67393; Santa Cruz Biotechnology, Dallas, TX, USA PAR3 Polarity protein Rabbit 1:50 11085-1-AP; Proteintech Group, Chicago, IL, USA aPKCλ Polarity protein Rabbit 1:100 Sc-11399; Santa Cruz Biotechnology β-catenin OLM marker Mouse 1:300 610153; BD Biosciences DIC Dynein intermediate chain Mouse 1:400 MAB1618; Millipore Open in a separate window List of Primary Antibodies Used in Immunohistochemistry

Techniques: Labeling, Staining, Immunoprecipitation, Control, Western Blot